Colloidal Coomassie Blue Stain
Colloidal Coomassie Blue Stain
Written by Guillaume Jacquemet
Stain Preparation:
In a large beaker add the ingredient in the order listed below
- Distilled H20 about 650 ml
- Ammonium Sulfate, 100g (final concentration 10%)
Make sure the Ammonium Sulfate is dissolved before adding the Coomassie G-250 solution to a final of 0.1%.
- Coomassie G-250 (Sigma: B0770), 20 ml of 5% (w/v) solution in H20
- Ortho-Phosphoric Acid, 30 ml
- Ethanol, 200 ml
- Bring final volume up to 1L with H20
Procedure:
- After SDS PAGE rinse gel twice with 100 ml distilled H20 (3 minutes each wash)
- Add Coomassie Blue working solution to gel and stain for at least 2 hours (for convenience this can be left to proceed overnight)
Bands should be visible without de-staining but you can de-stain with H20
Colloidal Coomassie Blue Stain – right hand column
