Colloidal Coomassie Blue Stain

Colloidal Coomassie Blue Stain

Written by Guillaume Jacquemet

Stain Preparation:

In a large beaker add the ingredient in the order listed below

  1. Distilled H20 about 650 ml
  2. Ammonium Sulfate, 100g (final concentration 10%)
    Make sure the Ammonium Sulfate is dissolved before adding the Coomassie G-250 solution to a final of 0.1%.
  3. Coomassie G-250 (Sigma: B0770), 20 ml of 5% (w/v) solution in H20
  4. Ortho-Phosphoric Acid, 30 ml
  5. Ethanol, 200 ml
  6. Bring final volume up to 1L with H20


  1. After SDS PAGE rinse gel twice with 100 ml distilled H20 (3 minutes each wash)
  2. Add Coomassie Blue working solution to gel and stain for at least 2 hours (for convenience this can be left to proceed overnight)

Bands should be visible without de-staining but you can de-stain with H20

Colloidal Coomassie Blue Stain – right hand column

Colloidal Coomassie Blue Stain