Publications

Highlight publications

Highlight publications

Nature Communication Articles: Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling AND A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting.

These studies further elaborate our knowledge of proteins that reside in the environment of adhesion complexes. Specifically they define the adhesion-induced repertoire of phosphorlyated proteins in response to integrin-fibronectin ligation and show that the activation-state of beta 1 integrins dictates the composition of protein complexes proximal to integrins.

Citation

Robertson J, Jacquemet G, Byron A, Jones MC, Warwood S, Selley JN, Knight D, Humphries JD, Humphries MJ. (2015). Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat. Commun. 6:6265doi: 10.1038/ncomms7265. Full text | PubMed entry
Byron, A., Askari, J.A., Humphries, J.D., Jacquemet, G., Koper, E.J., Warwood, S., Choi, C.K., Stroud, M.J., Chen, C.S., Knight, D., Humphries, M.J. (2015). A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nat. Commun. 6:6135 doi: 10.1038/ncomms7135. Full text | PubMed entry

A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting.Developmental Cell Articles: A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis AND Syndecan-4 Phosphorylation Is a Control Point for Integrin Recycling

These studies demonstrate that syndecan-4 regulates integrin trafficking by modulating their internalisation and recycling. These results shed new light on how syndecan modulate cell migration.

Citation

MR Morgan, H Hamidi, MD Bass, S Warwood, C Ballestrem, and MJ Humphries. (2012) Syndecan-4 Phosphorylation Is a Control Point for Integrin Recycling. Dev Cell. 287(33): 28027-36. Full text | PubMed entry

MD Bass, RC Williamson, RD Nunan, JD Humphries, A Byron, MR Morgan, P Martin, and MJ Humphries (2011) A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis. Dev. Cell. 21(4):681-93. Full text | PubMed entry

Journal of Cell Biology Article: integrin extension in focal adhesions

Focal adhesions are sites of integrin extension.This study provides strong evidence that the conformation of integrins in focal adhesions is extended, whereas unligated integrin is bent. These results highlight the importance of integrin receptor extension and leg domain separation in vivo.

Citation

JA Askari, CJ Tynan, SE Webb, ML Martin-Fernandez, C Ballestrem and MJ Humphries (2010) Focal adhesions are sites of integrin extension. J. Cell Biol. 188: 891-903.
Full text | PubMed entry

Further information

Cell Migration Gateway Featured Article: Integrin structure: Importance of extension

Faculty of 1000 Biology evaluation

Science Signaling Research Article: integrin proteomics

Science Signaling coverOur work describes a methodology for the isolation and proteomic analysis of ligand-induced integrin adhesion complexes. Using this approach, we identified regulator of chromosome condensation 2 (RCC2) as a component of fibronectin-activated integrin signalling pathways that regulate cell migration.

Citation

JD Humphries*, A Byron*, MD Bass, SE Craig, JW Pinney, D Knight and MJ Humphries (2009) Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal. 2: ra51. Full text | Reprint | PubMed entry

Further information

Press release: University of Manchester | EurekAlert | AlphaGalileo | ScienceDaily

Science Signaling Editor’s Summary: Integrin interactors

Science Signaling Perspective: Integrin proteomes reveal a new guide for cell motility

Science Signaling Editorial Guide: 2009: Signaling breakthroughs of the year

University of Manchester Faculty of Life Sciences highlight research publication

Faculty of 1000 Biology evaluation

* These authors contributed equally to this work.

Journal of Cell Biology Article: adhesion receptor cooperation

Adhesion receptor cooperationThis paper identifies p190RhoGAP as the convergence point for adhesive signals mediated by α5β1 integrin and syndecan-4 and explains the cooperation between extracellular matrix receptors during cell adhesion.

Citation

MD Bass, MR Morgan, KA Roach, J Settleman, AB Goryachev and MJ Humphries (2008) p190RhoGAP is the convergence point of adhesion signals from α5β1 integrin and syndecan-4. J. Cell Biol. 181: 1013-26. Full text | PubMed entry

Further information

Journal of Cell Biology In This Issue: Complementary receptors control RhoA

Faculty of 1000 Biology evaluation

Highlight publications

Highlight publications

Nature Communication Articles: Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling AND A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting.

These studies further elaborate our knowledge of proteins that reside in the environment of adhesion complexes. Specifically they define the adhesion-induced repertoire of phosphorlyated proteins in response to integrin-fibronectin ligation and show that the activation-state of beta 1 integrins dictates the composition of protein complexes proximal to integrins.

Citation

Robertson J, Jacquemet G, Byron A, Jones MC, Warwood S, Selley JN, Knight D, Humphries JD, Humphries MJ. (2015). Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat. Commun. 6:6265doi: 10.1038/ncomms7265. Full text | PubMed entry
Byron, A., Askari, J.A., Humphries, J.D., Jacquemet, G., Koper, E.J., Warwood, S., Choi, C.K., Stroud, M.J., Chen, C.S., Knight, D., Humphries, M.J. (2015). A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nat. Commun. 6:6135 doi: 10.1038/ncomms7135. Full text | PubMed entry

A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting.Developmental Cell Articles: A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis AND Syndecan-4 Phosphorylation Is a Control Point for Integrin Recycling

These studies demonstrate that syndecan-4 regulates integrin trafficking by modulating their internalisation and recycling. These results shed new light on how syndecan modulate cell migration.

Citation

MR Morgan, H Hamidi, MD Bass, S Warwood, C Ballestrem, and MJ Humphries. (2012) Syndecan-4 Phosphorylation Is a Control Point for Integrin Recycling. Dev Cell. 287(33): 28027-36. Full text | PubMed entry

MD Bass, RC Williamson, RD Nunan, JD Humphries, A Byron, MR Morgan, P Martin, and MJ Humphries (2011) A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis. Dev. Cell. 21(4):681-93. Full text | PubMed entry

Journal of Cell Biology Article: integrin extension in focal adhesions

Focal adhesions are sites of integrin extension.This study provides strong evidence that the conformation of integrins in focal adhesions is extended, whereas unligated integrin is bent. These results highlight the importance of integrin receptor extension and leg domain separation in vivo.

Citation

JA Askari, CJ Tynan, SE Webb, ML Martin-Fernandez, C Ballestrem and MJ Humphries (2010) Focal adhesions are sites of integrin extension. J. Cell Biol. 188: 891-903.
Full text | PubMed entry

Further information

Cell Migration Gateway Featured Article: Integrin structure: Importance of extension

Faculty of 1000 Biology evaluation

Science Signaling Research Article: integrin proteomics

Science Signaling coverOur work describes a methodology for the isolation and proteomic analysis of ligand-induced integrin adhesion complexes. Using this approach, we identified regulator of chromosome condensation 2 (RCC2) as a component of fibronectin-activated integrin signalling pathways that regulate cell migration.

Citation

JD Humphries*, A Byron*, MD Bass, SE Craig, JW Pinney, D Knight and MJ Humphries (2009) Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal. 2: ra51. Full text | Reprint | PubMed entry

Further information

Press release: University of Manchester | EurekAlert | AlphaGalileo | ScienceDaily

Science Signaling Editor’s Summary: Integrin interactors

Science Signaling Perspective: Integrin proteomes reveal a new guide for cell motility

Science Signaling Editorial Guide: 2009: Signaling breakthroughs of the year

University of Manchester Faculty of Life Sciences highlight research publication

Faculty of 1000 Biology evaluation

* These authors contributed equally to this work.

Journal of Cell Biology Article: adhesion receptor cooperation

Adhesion receptor cooperationThis paper identifies p190RhoGAP as the convergence point for adhesive signals mediated by α5β1 integrin and syndecan-4 and explains the cooperation between extracellular matrix receptors during cell adhesion.

Citation

MD Bass, MR Morgan, KA Roach, J Settleman, AB Goryachev and MJ Humphries (2008) p190RhoGAP is the convergence point of adhesion signals from α5β1 integrin and syndecan-4. J. Cell Biol. 181: 1013-26. Full text | PubMed entry

Further information

Journal of Cell Biology In This Issue: Complementary receptors control RhoA

Faculty of 1000 Biology evaluation

Top 20 publications

Top 20 publications

  1. MJ Humphries, K Olden and KM Yamada (1986) A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science 233: 467-470.
  2. MJ Humphries, SK Akiyama, A Komoriya, K Olden and KM Yamada (1986) Identification of an alternatively spliced site in human plasma fibronectin that mediates cell type-specific adhesion. J. Cell Biol. 103: 2637-2647.
  3. MJ Humphries, A Komoriya, SK Akiyama, K Olden and KM Yamada (1987) Identification of two distinct regions of the type III connecting segment of human plasma fibronectin that promote cell type-specific adhesion. J. Biol. Chem. 262: 6886-6892.
  4. MJ Humphries, KM Yamada and K Olden (1988) Investigation of the biological effects of anti-cell adhesive synthetic peptides that inhibit experimental metastasis of B16-F10 murine melanoma cells. J. Clin. Invest. 81: 782-790.
  5. EA Wayner, A Garcia-Pardo, MJ Humphries, JA McDonald and WG Carter (1989) Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J. Cell Biol. 109: 1321-1330.
  6. AP Mould, LA Wheldon, A Komoriya, EA Wayner, KM Yamada and MJ Humphries (1990) Affinity chromatographic isolation of the melanoma adhesion receptor for the IIICS region of fibronectin and its identification as the integrin α4β1. J. Biol. Chem. 265: 4020-4024.
  7. AP Mould, A Komoriya, KM Yamada and MJ Humphries (1991) The CS5 peptide is a second site in the IIICS region of fibronectin recognized by the integrin α4β1. Inhibition of α4β1 function by RGD peptide homologues. J. Biol. Chem. 266: 3579-3585.
  8. A Komoriya, LJ Green, M Mervic, SS Yamada, KM Yamada and MJ Humphries (1991) The minimal essential sequence for a major cell-type specific adhesion site (CS1) within the alternatively spliced IIICS domain of fibronectin is Leu-Asp-Val. J. Biol. Chem. 266: 15075-15079.
  9. AP Mould, SK Akiyama and MJ Humphries (1995) Regulation of integrin α5β1-fibronectin interactions by divalent cations: Evidence for distinct classes of binding sites for Mn2+, Mg2+ and Ca2+. J. Biol. Chem. 270: 26270-26277.
  10. AP Mould, SK Akiyama and MJ Humphries (1996) The inhibitory anti-β1 integrin monoclonal antibody 13 recognizes an epitope that is attenuated by ligand occupancy: Evidence for allosteric inhibition of integrin function. J. Biol. Chem. 271: 20365-20374.
  11. AP Mould, JA Askari, S Aota, KM Yamada, A Irie, Y Takada, HJ Mardon and MJ Humphries (1997) Defining the topology of integrin α5β1-fibronectin interactions using inhibitory anti-α5 and anti-β1 monoclonal antibodies: Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the α5 subunit. J. Biol. Chem. 272: 17283-17292.
  12. T Ng, D Shima, A Squire, PIH Bastiaens, S Gschmeissner, MJ Humphries and PJ Parker (1999) PKCα regulates β1 integrin-dependent cell motility through association and control of integrin traffic. EMBO J. 18: 3909-3923.
  13. AP Mould, JA Askari and MJ Humphries (2000) Molecular basis of ligand recognition by integrin α5β1. I. Specificity of ligand binding is determined by amino acid sequences in the second and third NH2-terminal repeats of the α subunit. J. Biol. Chem. 275: 20324-20336.
  14. AP Mould, JA Askari, S Barton, AD Kline, PA McEwan, SE Craig and MJ Humphries (2002) Integrin activation involves a conformational change in the α1 helix of the β subunit A-domain. J. Biol. Chem. 277: 19800-19805. Full text | PubMed entry
  15. Z Mostafavi-Pour, JA Askari, SJ Parkinson, PJ Parker, TTC Ng and MJ Humphries (2003) Integrin-specific signaling pathways controlling focal adhesion formation and cell migration. J. Cell Biol. 161: 155-167. Full text | PubMed entry
  16. AP Mould, SJ Barton, JA Askari, PA McEwan, PA Buckley, SE Craig and MJ Humphries (2003) Conformational changes in the integrin βA-domain provide a mechanism for signal transduction via hybrid domain movement. J. Biol. Chem. 278: 17028-17035. Full text | PubMed entry
  17. AP Mould, EJ Symonds, PA Buckley, JG Grossmann, PA McEwan, SJ Barton, JA Askari, SE Craig, J Bella and MJ Humphries (2003) Structure of an integrin-ligand complex deduced from solution x-ray scattering and site-directed mutagenesis. J. Biol. Chem. 278: 39993-9. Full text | PubMed entry
  18. MD Bass, KA Roach, MR Morgan, Z Mostafavi-Pour, T Schoen, T Muramatsu, U Mayer, C Ballestrem, JP Spatz and MJ Humphries (2007) Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix. J. Cell Biol. 177: 527-38. Full text | PubMed entry
  19. MD Bass, MR Morgan, KA Roach, J Settleman, AB Goryachev and MJ Humphries (2008) p190RhoGAP is the convergence point of adhesion signals from α5β1 integrin and syndecan-4. J. Cell Biol. 181: 1013-26. Full text | PubMed entry
  20. JD Humphries*, A Byron*, MD Bass, SE Craig, JW Pinney, D Knight and MJ Humphries (2009) Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal. 2: ra51. Full text | Reprint | PubMed entry

Free-access papers

Free-access papers

Robertson J, Jacquemet G, Byron A, Jones MC, Warwood S, Selley JN, Knight D, Humphries JD, Humphries MJ. (2015). Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat. Commun. 6:6265 doi: 10.1038/ncomms7265. Full text | PubMed entry

Byron, A., Askari, J.A., Humphries, J.D., Jacquemet, G., Koper, E.J., Warwood, S., Choi, C.K., Stroud, M.J., Chen, C.S., Knight, D., Humphries, M.J. (2015). A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nat. Commun. 6:6135doi: 10.1038/ncomms7135. Full text | PubMed entry

Ng, D.H., Humphries, J.D., Byron, A., Millon-Fremillon, A., & Humphries, M.J. (2014). Microtubule-dependent modulation of adhesion complex composition. PLOS ONE 9(12): e115213. Full text | PubMed entry

Mould, A.P., Craig, S.E., Byron, S.K., Humphries, M.J., & Jowitt T.A. (2014). Disruption of integrin-fibronectin complexes by allosteric but not ligand-mimetic inhibitors. Biochem. J. 464(3): 301-13. Full text | PubMed entry

Lennon, R., Randles, M. & Humphries, M.J. (2014). The importance of podocyte adhesion for a healthy glomerulus. Front. Endocrinol. (Lausanne). 5(160): eCollection 2014. Full text | PubMed entry

Byron, A., Randles, M., Humphries, J.D., Mironov, A., Hamidi, H., Harris, S., Mathieson, P., Saleem, M., Satchell, S., Zent, R., Humphries, M.J. & Lennon, R. (2014). Glomerular cell cross-talk influences composition and assembly of extracellular matrix. J. Am. Soc. Nephrol. 25(5): 953-66. Full text

Lennon, R., Byron, A., Humphries, J.D., Randles, M., Carisey, A., Murphy, S., Knight, D., Brenchley, P., Zent, R. & Humphries, M.J. (2014). Global analysis reveals the complexity of the human glomerular extracellular matrix. J. Am. Soc. Nephrol. 25(5): 939-51. Full text

Pritchett J, Athwal VS, Harvey E, Martin K, Llewellyn J, Ireland P, Nicolaides A, Humphries MJ, Bobola N, Hanley NA, Piper Hanley K. (2014) Epimorphin alters the inhibitory effects of SOX9 on Mmp13 in activated hepatic stellate cells. PLOS ONE. 27;9(6): e100091. Full text | PubMed entry

Warwood S, Byron A, Humphries MJ, Knight D. (2013). The effect of peptide adsorption on signal linearity and a simple approach to improve reliability of quantification. J. Proteomics 85: 160-4. Full text | PubMed entry

Seong J, Tajik A, Sun J, Guan JL, Humphries MJ, Craig SE, Shekaran A, García AJ, Lu S, Lin MZ, Wang N, Wang Y. (2013). Distinct biophysical mechanisms of focal adhesion kinase mechanoactivation by different extracellular matrix proteins. PNAS(USA). 110(48): 19372-7. Full text | PubMed entry

Jacquemet, G., Humphries, M. & Caswell, P (2013). Role of adhesion receptor trafficking in 3D cell migration. Curr Opin Cell Biol. 25(5): 627-32. Full text | PubMed entry

Jacquemet, G., Green, D., Bridgewater, R., von Kriegsheim, A., Humphries, M., Norman, J. & Caswell, P (2013). RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1-IQGAP1 complex. J. Cell Biol. 202(6): 917-35. Full text | PubMed entry

Jacquemet, G., Morgan, M., Byron, A., Humphries, J.D., Choi, C., Chen, C., Caswell, P. & Humphries, M (2013). Rac1 is deactivated at integrin activation sites through an IQGAP1-filamin-A-RacGAP1 pathway. J. Cell Sci. 126(Pt 18): 4121-35 Full text

Guillaume Jacquemet and Martin Humphries. (2013) IQGAP1 is a key node within the small GTPase network. Small GTPases 4(4): 199-207. Full text | PubMed entry

A Byron, Z li, JD Humphries, and MJ Humphries. (2013) Defining the extracellular matrix using proteomics. Int J Exp Pathol. 287(33): 28027-36. Full text | PubMed entry

F Kong, Z li, WM Park, DW Dumbauld, AJ García, AP Mould, MJ Humphries, and C Zhu. (2013) Cyclic Mechanical Reinforcement of Integrin-Ligand Interactions. Mol Cell. 287(33): 28027-36. Full text | PubMed entry

GL Lin, DM Cohen, RA Desai, MT Breckenridge, L Gao, MJ Humphries and CS Chen. (2013) Activation of beta 1 but not beta 3 integrin increases cell traction forces. FEBS Lett. Full text | PubMed entry

MM Le Goff, MJ Sutton, M Slevin, A Latif, MJ humphries, and PN Bishop. (2012) Opticin exerts its anti-angiogenic activity by regulating extracellular matrix adhesiveness. J Biol Chem. 287(33): 28027-36. Full text | PubMed entry

A Byron, JD Humphries, SE Craig, D Knight, and MJ Humphries (2012) Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment. Proteomics 12: 2107-14. Full text | PubMed entry

A Byron, JD Humphries, and MJ Humphries (2012) Alternative cellular roles for proteins identified using proteomics. J. Proteomics 75: 4184-5. Full text | PubMed entry

DA Tonge, HT de Burgh, R Docherty, MJ Humphries, SE Craig, and J Pizzey (2012) Fibronectin supports neurite outgrowth and axonal regeneration of adult brain neurons in vitro. Brain Res. 11: 4052-64. Full text | PubMed entry

ST Rashid, JD Humphries, A Byron, JD Humphries, A Byron, A Dhar, JA Askari, JN Selley, D Knight, RD Goldin, M Thursz, and MJ Humphries (2012) Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver. Dev. Cell. 21(4):681-93. Full text | PubMed entry

MD Bass, RC Williamson, RD Nunan, JD Humphries, A Byron, MR Morgan, P Martin, and MJ Humphries (2011) A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis. Dev. Cell. 21(4):681-93. Full text | PubMed entry

A Byron, JD Humphries, MD Bass, D Knight and MJ Humphries (2011) Proteomic analysis of integrin adhesion complexes. Sci. Signal. 4: pt2. Full text | Reprint | PubMed entry

HC Siebert, M Burg-Roderfeld, T Eckert, S Stötzel, U Kirch, T Diercks, MJ Humphries, M Frank, R Wechselberger, E Tajkhorshid and S Oesser (2010) Interaction of the α2A domain of integrin with small collagen fragments. Protein Cell 1: 393-495. Full text | PubMed entry

DC Worth, K Hodivala-Dilke, SD Robinson, SJ King, PE Morton, FB Gertler, MJ Humphries and M Parsons (2010) αvβ3 integrin spatially regulates VASP and RIAM to control adhesion dynamics and migration. J. Cell Biol. 189: 369-383. Full text | PubMed entry

T Schubert, J Schlegel, R Schmid, A Opolka, S Grassel, M Humphries and AK Bosserhoff (2010) Modulation of cartilage differentiation by melanoma inhibiting activity/cartilage-derived retinoic acid-sensitive protein (MIA/CD-RAP). Exp. Mol. Med. 42: 166-174. Full text | PubMed entry

JA Askari, CJ Tynan, SE Webb, ML Martin-Fernandez, C Ballestrem and MJ Humphries (2010) Focal adhesions are sites of integrin extension. J. Cell Biol. 188: 891-903. Full text | PubMed entry

A Byron, JD Humphries, JA Askari, SE Craig, AP Mould and MJ Humphries (2009) Anti-integrin monoclonal antibodies. J. Cell Sci. 122: 4009-11. Full text | PubMed entry

JD Humphries*, A Byron*, MD Bass, SE Craig, JW Pinney, D Knight and MJ Humphries (2009) Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal. 2: ra51. Full text | Reprint | PubMed entry

F Kong, AJ García, AP Mould, MJ Humphries and C Zhu (2009) Demonstration of catch bonds between an integrin and its ligand. J. Cell Biol. 185: 1275-84. Full text | PubMed entry

C Faye, C Moreau, E Chautard, R Jetne, N Fukai, F Ruggiero, MJ Humphries, BR Olsen and S Ricard-Blum (2009) Molecular interplay between endostatin, integrins, and heparan sulfate. J. Biol. Chem. 284: 22029-40. Full text | PubMed entry

NO Deakin*, MD Bass*, S Warwood, J Schoelermann, Z Mostafavi-Pour, D Knight, C Ballestrem and MJ Humphries (2009) An integrin-α4-14-3-3ζ-paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration. J. Cell Sci. 122: 1654-64. Full text | PubMed entry

D Valdembri, PT Caswell, KI Anderson, JP Schwarz, I König, E Astanina, F Caccavari, JC Norman, MJ Humphries, F Bussolino and G Serini (2009) Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol. 7: e25. Full text | PubMed entry

CM Mahoney, MR Morgan, A Harrison, MJ Humphries and MD Bass (2009) Therapeutic ultrasound bypasses canonical syndecan-4 signaling to activate rac1. J. Biol. Chem. 284: 8898-909. Full text | PubMed entry

JA Askari, PA Buckley, AP Mould and MJ Humphries (2009) Linking integrin conformation to function. J. Cell Sci. 122: 165-70. Full text | PubMed entry

D Valdramidou, MJ Humphries and AP Mould (2008) Distinct roles of β1 metal ion-dependent adhesion site (MIDAS), adjacent to MIDAS (ADMIDAS), and ligand-associated metal-binding site (LIMBS) cation-binding sites in ligand recognition by integrin α2β1. J. Biol. Chem. 283: 32704-14. Full text | PubMed entry

MD Bass, MR Morgan, KA Roach, J Settleman, AB Goryachev and MJ Humphries (2008) p190RhoGAP is the convergence point of adhesion signals from α5β1 integrin and syndecan-4. J. Cell Biol. 181: 1013-26. Full text | PubMed entry

D Telci, Z Wang, X Li, EAM Verderio, MJ Humphries, M Baccarini, H Basaga and M Griffin (2008) Fibronectin-tissue transglutaminase matrix rescues RGD-impaired cell adhesion through syndecan-4 and β1 integrin co-signaling. J. Biol. Chem. 283: 20937-47. Full text | PubMed entry

M Parsons, AJ Messent, JD Humphries, NO Deakin and MJ Humphries (2008) Quantification of integrin receptor agonism by fluorescence lifetime imaging. J. Cell Sci. 121: 265-71. Full text | PubMed entry

JD Humphries, P Wang, C Streuli, B Geiger, MJ Humphries and C Ballestrem (2007) Vinculin controls focal adhesion formation by direct interactions with talin and actin. J. Cell Biol. 179: 1043-57. Full text | PubMed entry

PT Caswell, HJ Spence, M Parsons, DP White, K Clark, KW Cheng, GB Mills, et al. (2007) Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments. Dev. Cell 13: 496-510. Full text | PubMed entry

MD Bass, KA Roach, MR Morgan, Z Mostafavi-Pour, T Schoen, T Muramatsu, U Mayer, C Ballestrem, JP Spatz and MJ Humphries (2007) Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix. J. Cell Biol. 177: 527-38. Full text | PubMed entry

DV Bax, Y Mahalingam, S Cain, K Mellody, L Freeman, K Younger, CA Shuttleworth, MJ Humphries, JR Couchman and CM Kielty (2007) Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation. J. Cell Sci. 120: 1383-92. Full text | PubMed entry

JD Humphries and MJ Humphries (2007) CD14 is a ligand for the integrin α4β1. FEBS Lett. 581: 757-63. Full text | PubMed entry

MD Bass, MR Morgan and MJ Humphries (2007) Integrins and syndecan-4 make distinct, but critical, contributions to adhesion contact formation. Soft Matter 3: 372-6. Full text | PubMed entry

ES Wijelath, S Rahman, M Namekata, J Murray, T Nishimura, Z Mostafavi-Pour, Y Patel, Y Suda, MJ Humphries and M Sobel (2006) Heparin-II domain of fibronectin is a vascular endothelial growth factor-binding domain: enhancement of VEGF biological activity by a singular growth factor/matrix protein synergism. Circ. Res. 99: 853-60. Full text | PubMed entry

JD Humphries*, A Byron* and MJ Humphries (2006) Integrin ligands at a glance. J. Cell Sci. 119: 3901-3. Full text | PubMed entry

RN Sumathipala, C Xu, J Seago, AP Mould, MJ Humphries, SE Craig, Y Patel, ES Wijelath, M Sobel and S Rahman (2006) The “linker” region (amino acids 38-47) of the disintegrin elegantin is a novel inhibitory domain of integrin α5β1-dependent cell adhesion on fibronectin: evidence for the negative regulation of fibronectin synergy site biological activity. J. Biol. Chem. 281: 37686-96. Full text | PubMed entry

NJ Turner, MO Murphy, CM Kielty, CA Shuttleworth, RA Black, MJ Humphries, MG Walker and AE Canfield (2006) α2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an α2β1 integrin-dependent mechanism: an in vitro and in vivo study. Circulation 114: 820-9. Full text | PubMed entry

AP Mould, JA McLeish, J Huxley-Jones, AC Goonesinghe, AF Hurlstone, RP Boot-Handford and MJ Humphries MJ (2006) Identification of multiple integrin beta1 homologs in zebrafish (Danio rerio). BMC Cell Biol. 7: 24. Full text | PubMed entry

R Bauer, M Humphries, R Fässler, A Winklmeier, SE Craig and AK Bosserhoff (2006) Regulation of integrin activity by MIA. J. Biol. Chem. 281: 11669-77. Full text | PubMed entry

J Gläsner, H Blum, V Wehner, HU Stilz, JD Humphries, GP Curley, AP Mould, MJ Humphries, R Hallmann, M Röllinghoff and A Gessner (2005) A small molecule α4β1 antagonist prevents development of murine lyme arthritis without affecting protective immunity. J. Immunol. 175: 4724-34. PubMed entry

X Meng, K Cheng, O Krohkin, AP Mould, MJ Humphries, W Ens, K Standing and JA Wilkins (2005) Evidence for the presence of a low-mass β1 integrin on the cell surface. J. Cell Sci. 118: 4009-16. Full text | PubMed entry

R Ewan, J Huxley-Jones, AP Mould, MJ Humphries, DL Robertson and RP Boot-Handford (2005) The integrins of the urochordate Ciona intestinalis provide novel insights into the molecular evolution of the vertebrate integrin family. BMC Evol. Biol. 5: 31. Full text | PubMed entry

J Bella and MJ Humphries (2005) Cα-HO=C hydrogen bonds contribute to the specificity of RGD cell-adhesion interactions. BMC Struct. Biol. 5: 4. Full text | PubMed entry

Y Wei, RP Czekay, L Robillard, MC Kugler, F Zhang, KK Kim, JP Xiong, MJ Humphries and HA Chapman (2005) Regulation of α5β1 integrin conformation and function by urokinase receptor binding. J. Cell Biol. 168: 501-11. Full text | PubMed entry

SL Dallas, P Sivakumar, CJ Jones, Q Chen, DM Peters, DF Mosher, MJ Humphries and CM Kielty (2005) Fibronectin regulates latent transforming growth factor-β (TGFβ) by controlling matrix assembly of latent TGFβ-binding protein-1. J. Biol. Chem. 280: 18871-80. Full text | PubMed entry

JD Humphries, NR Schofield, Z Mostafavi-Pour, LJ Green, AN Garratt, AP Mould and MJ Humphries (2005) Dual functionality of the anti-β1 integrin antibody, 12G10, exemplifies agonistic signalling from the ligand binding pocket of integrin adhesion receptors. J. Biol. Chem. 280: 10234-43. Full text | PubMed entry

K Clark, R Pankov, MA Travis, JA Askari, AP Mould, SE Craig, P Newham, KM Yamada and MJ Humphries (2005) A specific α5β1-integrin conformation promotes directional integrin translocation and fibronectin matrix formation. J. Cell Sci. 118: 291-300. Full text | PubMed entry

AP Mould, MA Travis, SJ Barton, JA Hamilton, JA Askari, SE Craig, PR Macdonald, RA Kammerer, PA Buckley and MJ Humphries (2005) Evidence that monoclonal antibodies directed against the integrin β subunit plexin/semaphorin/integrin domain stimulate function by inducing receptor extension. J. Biol. Chem. 280: 4238-46. Full text | PubMed entry

X Zhang, SE Craig, H Kirby, MJ Humphries and VT Moy (2004) Molecular basis for the dynamic strength of the integrin α4β1/VCAM-1 Interaction. Biophys. J. 87: 3470-8. Full text | PubMed entry

MJ Humphries, MA Travis, K Clark and AP Mould (2004) Mechanisms of integration of cells and extracellular matrices by integrins. Biochem. Soc. Trans. 32: 822-825. PubMed entry

MA Travis, A Van Der Flier, RA Kammerer, AP Mould, A Sonnenberg and MJ Humphries (2004) Interaction of filamin A with the integrin β7 cytoplasmic domain: role of alternative splicing and phosphorylation. FEBS Lett. 569: 185-90. Full text | PubMed entry

MJ Humphries (2004) Monoclonal antibodies as probes of integrin priming and activation. Biochem. Soc. Trans. 32: 407-11. Full text | PubMed entry

SJ Barton, MA Travis, JA Askari, PA Buckley, SE Craig, MJ Humphries and AP Mould (2004) Novel activating and inactivating mutations in the integrin β1 subunit A domain. Biochem. J. 380: 401-7. Full text | PubMed entry

DV Bax, AJ Messent, J Tart, M Van Hoang, J Kott, RA Maciewicz and MJ Humphries (2004) Integrin α5β1 and TNFα converting enzyme (TACE/ADAM-17) interact in vitro and colocalise in migrating HeLa cells. J. Biol. Chem. 279: 22377-86. Full text | PubMed entry

*These authors contributed equally to this work.

Full publication list

Full list of publications

Horton ER, Astudillo P, Humphries MJ, Humphries JD. (2016) Mechanosensitivity of integrin adhesion complexes: role of the consensus adhesome.Exp Cell Res. 10;343(1):7-13. PubMed

Ajeian, J.N., Horton, E.R., Astudillo, P., Byron, A., Askari, J.A., Millon-Frémillon, A., Knight, D., Kimber, S.J., Humphries, M.J. and Humphries, J.D. (2016) Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl. 10, 51-7. PubMed

Horton, E.R., Humphries, J.D., Stutchbury, B., Jacquemet, G., Ballestrem, C., Barry, S.T. and Humphries, M.J. (2016) Modulation of FAK and Src adhesion signalling occurs independently of adhesion complex composition. J Cell Biol. 212, 349-64. PubMed

Byron, A., Askari, J.A., Humphries, J.D., Jacquemet, G., Koper, E.J., Warwood, S., Choi, C.K., Stroud, M.J., Chen, C.S., Knight, D. and Humphries, M.J. (2015). A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nature Comm. 6, 6135. PubMed

Horton, E.R., Byron, A., Askari, J.A., Ng, D.H.J., Millon-Frémillon, A., Robertson, J., Koper, E.J., Paul, N.R., Warwood, S., Knight, D., Humphries, J.D. and Humphries, M.J. (2015). Definition of a consensus integrin adhesome and analysis of its dynamics during adhesion complex assembly and disassembly. Nat Cell Biol. 17, 1577-87. PubMed

Humphries, J.D., Paul, N.R., Humphries, M.J. and Morgan, M.R. (2015). Emerging properties of adhesion complexes: What are they and what do they do? Trends Cell Biol. 25, 388-97. PubMed

Jones, M.C., Humphries, J.D., Byron, A., Millon-Frémillon, A., Robertson, J., Paul, N.R., Ng, D.H.J., Askari, J.A. and Humphries, M.J. (2015). Isolation of integrin-based adhesion complexes. Curr Protoc Cell Biol. 66, 9.8.1-9.8.15. PubMed

Randles, M.J., Woolf, A.S., Huang, J.L., Byron, A., Jonathan, D., Humphries, J.D., Price, K.L., Kolatsi-Joannou, M., Collinson, S., Denny, T., Knight, D., Mironov, A., Starborg, T., Korstanje, R., Humphries, M.J., Long, D.A. and Lennon, R. (2015). Genetic background is a key determinant of glomerular extracellular matrix composition and organization. J Am Soc Nephr. 26, 3021-34. PubMed

Robertson, R., Jacquemet, G., Humphries, J.D., Byron, A., Jones, M., Warwood, S., Selley, J., Knight, D. and Humphries, M.J. (2015). Defining the phospho-adhesome: phosphoproteomic analysis of integrin signalling. Nature Commun. 6, 6265. PubMed

Byron, A., Randles, M.J., Humphries, J.D., Mironov, A., Hamidi, H., Harris, S., Mathieson, P.W., Saleem, M.A., Satchell, S.S., Zent, R., Humphries, M.J. and Lennon, R.(2014). Glomerular cell crosstalk influences composition and assembly of extracellular matrix. J Am Soc Nephrol. 25, 953-66. ePub

Lennon, R., Byron, A., Humphries, J.D., Randles, M.J., Carisey, A., Murphy, S., Knight, D., Brenchley, P.E., Zent, R. and Humphries, M.J. (2014). Global analysis reveals the complexity of the human glomerular extracellular matrix. J. Am. Soc. Nephrol. ePub

Mould, A.P., Craig, S.E., Byron, S.K., Humphries, M.J. and Jowitt, T.A. (2014). Disruption of integrin-fibronectin complexes by allosteric but not ligand-mimetic inhibitors. Biochem J. 464, 301-313. PubMed

Ng, D., Humphries, J.D., Byron, A., Millon-Fremillon, A., Warwood, S., Knight, D. and Humphries, M.J. Microtubule-dependent modulation of adhesion complex composition and location. eCollection. 9: e115213. PubMed

Jacquemet, G., Green, D.M., Bridgewater, R.M., von Kriegsheim, A., Humphries, M.J., Norman, J.C. and Caswell, P.T. (2013). RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1-IQGAP1 complex. J Cell Biol. 202, 917-35. PubMed

Byron, A., Humphries, J.D., Humphries, M.J. (2013). Defining the extracellular matrix using proteomics. Int. J. Exp. Pathol. Epub ahead of print

Jacquemet, G. and Humphries, M.J. (2013). IQGAP1 is a key node within the small GTPase network. Brief report, Small GTPases. 19, 4. PubMed

Jacquemet, G., Humphries, M.J. and Caswell, P.T. (2013). Role of adhesion receptor trafficking in 3D cell migration. 10.1016/j.ceb.2013.05.008 Curr Opin Cell Biol. PMC

Jacquemet, G., Morgan, M.R., Byron, A., Humphries, J.D., Choi, C.K., Chen, C.S., Caswell, P.T. and Humphries, M.J. (2013). Rac1 is deactivated at integrin activation sites via an IQGAP1/filamin-A/RacGAP1 pathway. J Cell Sci. in press.

Kong, F., Parks, W.M., Dumbauld, D.W., Takagi, J., Garcia, A.J., Mould, A.P., Humphries, M.J. and Zhu, C. (2013). Cyclic mechanical reinforcement of integrin-ligand interactions. Mol. Cell 49, 1060-8. sciencedirect

Soteriou, D., Iskender, B., Byron, A., Humphries, J.D., Borg-Bartolo, S., Haddock, M.C., Baxter, M.A., Knight, D., Humphries, M.J., Kimber, S.J. (2013). Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance. J. Biol. Chem. http://dx.doi.org/10.1074/jbc.M113.463372

Warwood, S., Byron, A., Humphries, M.J. and Knight, D. (2013). The effect of peptide adsorption on signal linearity and a simple approach to improve reliability of quantification. J Proteomics. 85,160-4. Sciencedirect

Morgan, M.R., Hamidi, H., Bass, M.D., Warwood, S., Ballestrem C. and Humphries, M.J. (2013). Syndecan-4 phosphorylation is a control point for integrin recycling. Dev. Cell 24, 472-85. PubMed

Byron, A., Humphries, J.D., Craig, S.E., Knight, D and Humphries, M.J. (2012). Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment. Proteomics 12, 2107-14. doi: 10.1002/pmic.201100487. PubMed

Byron, A., Humphries, J.D. and Humphries, M.J. (2012). Alternative cellular roles for proteins identified using proteomics. J. Proteomics 75, 4184-5. PubMed

Le Goff, M.M., Sutton, M.J., Slevin, M., Latif, A., Humphries, M.J. and Bishop, P.N. (2012). Opticin exerts its anti-angiogenic activity by regulating extracellular matrix adhesiveness. J. Biol. Chem. 287, 28027-28036. PubMed

Rashid, S.T., Humphries, J.D., Byron, A., Dhar, A., Askari, J.A., Selley, J.N., Knight, D., Goldin, R.D., Thursz, M., Humphries, M.J. (2012). Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver. J Proteome Res11, 4052-64.

Tonge, D.A., de Burgh, H.T., Docherty, R., Humphries, M.J., Craig, S.E. and Pizzey, J. (2012) Fibronectin supports neurite outgrowth and axonal regeneration of adult brain neurons in vitro. Brain Res. 1453, 8-16. PubMed

Bass, M.D., Williamson, R.C., Humphries, J.D., Byron, A., Morgan, M.R., Martin P. and Humphries, M.J. (2011). A syndecan-4 hair trigger initiates wound healing through caveolin- and RhoG-regulated integrin endocytosis. Dev. Cell 21, 681-93. PubMed

Byron, A., Humphries, J. D., Bass, M. D., Knight, D., & Humphries, M. J. (2011). Proteomic analysis of integrin adhesion complexes. Science Signaling, 4(167), pt2. doi:10.1126/scisignal.2001827. Sci. Signal. 4: pt2

Rantala, J.K., Pouwels, J., Pellinen, T., Veltel, S., Laasola, P., Potter, C.S., Duffy, T., Sundberg, J.P., Kallioniemi, O., Askari, J.A., Humphries, M., Parsons, M., Salmi, M. and Ivaska, J. (2011). SHARPIN is an endogenous inhibitor of beta1-integrin activation. Nature Cell Biol 13, 1315-24. doi: 10.1038/ncb2340. PubMed link

Tiwari, S., Askari, J.A., Humphries, M.J. and Bulleid, N.J. (2011). Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J. Cell Sci. 124: 1672-1680. PubMed link

Worth, D. C., Hodivala-Dilke, K., Robinson, S. D., King, S. J., Morton, P. E., Gertler, F. B., Humphries, M. J., and Parsons, M. (2010) Alpha v beta3 integrin spatially regulates VASP and RIAM to control adhesion dynamics and migration, J Cell Biol 189, 369-83 PubMed link.

Askari, J. A., Tynan, C. J., Webb, S. E., Martin-Fernandez, M. L., Ballestrem, C., and Humphries, M. J. (2010) Focal adhesions are sites of integrin extension, J Cell Biol 188, 891-903 PubMed link.

Mould, A. P., Koper, E. J., Byron, A., Zahn, G., and Humphries, M. J. (2009) Mapping the ligand-binding pocket of integrin alpha5beta1 using a gain-of-function approach, Biochem J 424, 179-89 PubMed link.

Morgan, M. R., Byron, A., Humphries, M. J., and Bass, M. D. (2009) Giving off mixed signals–distinct functions of alpha5beta1 and alphavbeta3 integrins in regulating cell behaviour, IUBMB Life 61, 731-8 PubMed link.

Mahoney, C. M., Morgan, M. R., Harrison, A., Humphries, M. J., and Bass, M. D. (2009) Therapeutic ultrasound bypasses canonical syndecan-4 signaling to activate rac1, J Biol Chem 284, 8898-909 PubMed link.

Kong, F., Garcia, A. J., Mould, A. P., Humphries, M. J., and Zhu, C. (2009) Demonstration of catch bonds between an integrin and its ligand, J Cell Biol 185, 1275-84 PubMed link.

Humphries, J. D., Byron, A., Bass, M. D., Craig, S. E., Pinney, J. W., Knight, D., and Humphries, M. J. (2009) Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6, Sci Signal 2, ra51 PubMed link.

Humphries, M. J., and Reynolds, A. (2009) Cell-to-cell contact and extracellular matrix, Curr Opin Cell Biol 21, 613-5 PubMed link.

Humphries, M. J. (2009) Cell adhesion assays, Methods Mol Biol 522, 1-8 PubMed link.

Faye, C., Moreau, C., Chautard, E., Jetne, R., Fukai, N., Ruggiero, F., Humphries, M. J., Olsen, B. R., and Ricard-Blum, S. (2009) Molecular interplay between endostatin, integrins, and heparan sulfate, J Biol Chem 284, 22029-40 PubMed link.

Deakin, N. O., Bass, M. D., Warwood, S., Schoelermann, J., Mostafavi-Pour, Z., Knight, D., Ballestrem, C., and Humphries, M. J. (2009) An integrin-α4-14-3-3ζ-paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration, J Cell Sci 122, 1654-64 PubMed link.

Byron, A., Humphries, J. D., Askari, J. A., Craig, S. E., Mould, A. P., and Humphries, M. J. (2009) Anti-integrin monoclonal antibodies, J Cell Sci 122, 4009-11 PubMed link.

Bass, M. D., Morgan, M. R., and Humphries, M. J. (2009) Syndecans shed their reputation as inert molecules, Sci Signal 2, pe18 PubMed link.

Askari, J. A., Buckley, P. A., Mould, A. P., and Humphries, M. J. (2009) Linking integrin conformation to function, J Cell Sci 122, 165-70 PubMed link.

Valdramidou, D., Humphries, M. J., and Mould, A. P. (2008) Distinct roles of beta 1 midas, admidas and limbs cation-binding sites in ligand recognition by integrin alpha 2beta 1, The Journal of biological chemistry PubMed link.

Telci, D., Wang, Z., Li, X., Verderio, E. A., Humphries, M. J., Baccarini, M., Basaga, H., and Griffin, M. (2008) Fibronectin-TG2 matrix rescues RGD-impaired cell adhesion through syndecan-4 and beta 1 integrin co-signaling, The Journal of biological chemistry PubMed link.

Parsons, M., Messent, A. J., Humphries, J. D., Deakin, N. O., and Humphries, M. J. (2008) Quantification of integrin receptor agonism by fluorescence lifetime imaging, Journal of cell science 121, 265-71 PubMed link.

Kloepper, J. E., Hendrix, S., Bodo, E., Tiede, S., Humphries, M. J., Philpott, M. P., Fassler, R., and Paus, R. (2008) Functional role of beta 1 integrin-mediated signalling in the human hair follicle, Experimental cell research 314, 498-508 PubMed link.

Humphries, S. M., Lu, Y., Canty, E. G., and Kadler, K. E. (2008) Active negative control of collagen fibrillogenesis in vivo. Intracellular cleavage of the type I procollagen propeptides in tendon fibroblasts without intracellular fibrils, J Biol Chem 283, 12129-35 PubMed link.

Bass, M. D., Morgan, M. R., Roach, K. A., Settleman, J., Goryachev, A. B., and Humphries, M. J. (2008) p190RhoGAP is the convergence point of adhesion signals from alpha 5 beta 1 integrin and syndecan-4, J Cell Biol 181, 1013-26 PubMed link.

Watson, P. M., Humphries, M. J., Relton, J., Rothwell, N. J., Verkhratsky, A., and Gibson, R. M. (2007) Integrin-binding RGD peptides induce rapid intracellular calcium increases and MAPK signaling in cortical neurons, Molecular and cellular neurosciences 34, 147-54 PubMed link.

Morgan, M. R., Humphries, M. J., and Bass, M. D. (2007) Synergistic control of cell adhesion by integrins and syndecans, Nature reviews 8, 957-69 PubMed link.

Kloepper, J. E., Hendrix, S., Bodo, E., Tiede, S., Humphries, M. J., Philpott, M. P., Fassler, R., and Paus, R. (2007) Functional role of beta1 integrin-mediated signalling in the human hair follicle, Exp Cell Res PubMed link.

Humphries, J. D., Wang, P., Streuli, C., Geiger, B., Humphries, M. J., and Ballestrem, C. (2007) Vinculin controls focal adhesion formation by direct interactions with talin and actin, The Journal of cell biology 179, 1043-57 PubMed link.

Humphries, J. D., and Humphries, M. J. (2007) CD14 is a ligand for the integrin alpha4beta1, FEBS letters 581, 757-63 PubMed link.

Bax, D. V., Mahalingam, Y., Cain, S., Mellody, K., Freeman, L., Younger, K., Shuttleworth, C. A., Humphries, M. J., Couchman, J. R., and Kielty, C. M. (2007) Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation, Journal of cell science 120, 1383-92 PubMed link.

Bass, M. D., Roach, K. A., Morgan, M. R., Mostafavi-Pour, Z., Schoen, T., Muramatsu, T., Mayer, U., Ballestrem, C., Spatz, J. P., and Humphries, M. J. (2007) Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix, The Journal of cell biology 177, 527-38 PubMed link.

Askari, J. A., Thornton, D. J., Humphries, J. D., Buckley, P. A., and Humphries, M. J. (2007) The alternatively spliced type III connecting segment of fibronectin is a zinc-binding module, Matrix Biol 26, 485-93 PubMed link.

Wijelath, E. S., Rahman, S., Namekata, M., Murray, J., Nishimura, T., Mostafavi-Pour, Z., Patel, Y., Suda, Y., Humphries, M. J., and Sobel, M. (2006) Heparin-II domain of fibronectin is a vascular endothelial growth factor-binding domain: enhancement of VEGF biological activity by a singular growth factor/matrix protein synergism, Circulation research 99, 853-60 PubMed link.

Turner, N. J., Murphy, M. O., Kielty, C. M., Shuttleworth, C. A., Black, R. A., Humphries, M. J., Walker, M. G., and Canfield, A. E. (2006) Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study, Circulation 114, 820-9 PubMed link.

Sumathipala, R., Xu, C., Seago, J., Mould, A. P., Humphries, M. J., Craig, S. E., Patel, Y., Wijelath, E. S., Sobel, M., and Rahman, S. (2006) The “linker” region (amino acids 38-47) of the disintegrin elegantin is a novel inhibitory domain of integrin alpha5beta1-dependent cell adhesion on fibronectin: evidence for the negative regulation of fibronectin synergy site biological activity, The Journal of biological chemistry 281, 37686-96 PubMed link.

Mould, A. P., McLeish, J. A., Huxley-Jones, J., Goonesinghe, A. C., Hurlstone, A. F., Boot-Handford, R. P., and Humphries, M. J. (2006) Identification of multiple integrin beta1 homologs in zebrafish (Danio rerio), BMC cell biology 7, 24 PubMed link.

Humphries, M. J., Limesand, K. H., Schneider, J. C., Nakayama, K. I., Anderson, S. M., and Reyland, M. E. (2006) Suppression of apoptosis in the protein kinase Cdelta null mouse in vivo, J Biol Chem 281, 9728-37 PubMed link.

Humphries, J. D., Byron, A., and Humphries, M. J. (2006) Integrin ligands at a glance, Journal of cell science 119, 3901-3 PubMed link.

Bauer, R., Humphries, M., Fassler, R., Winklmeier, A., Craig, S. E., and Bosserhoff, A. K. (2006) Regulation of integrin activity by MIA, The Journal of biological chemistry 281, 11669-77 PubMed link.

Wei, Y., Czekay, R. P., Robillard, L., Kugler, M. C., Zhang, F., Kim, K. K., Xiong, J. P., Humphries, M. J., and Chapman, H. A. (2005) Regulation of alpha5beta1 integrin conformation and function by urokinase receptor binding, J Cell Biol 168, 501-11 PubMed link.

Mould, A. P., Travis, M. A., Barton, S. J., Hamilton, J. A., Askari, J. A., Craig, S. E., Macdonald, P. R., Kammerer, R. A., Buckley, P. A., and Humphries, M. J. (2005) Evidence that monoclonal antibodies directed against the integrin beta subunit plexin/semaphorin/integrin domain stimulate function by inducing receptor extension, J Biol Chem 280, 4238-46 PubMed link.

Meng, X., Cheng, K., Krohkin, O., Mould, A. P., Humphries, M. J., Ens, W., Standing, K., and Wilkins, J. A. (2005) Evidence for the presence of a low-mass beta1 integrin on the cell surface, J Cell Sci 118, 4009-16 PubMed link.

Humphries, M. J., Mostafavi-Pour, Z., Morgan, M. R., Deakin, N. O., Messent, A. J., and Bass, M. D. (2005) Integrin-syndecan cooperation governs the assembly of signalling complexes during cell spreading, Novartis Found Symp 269, 178-88; discussion 188-92, 223-30 PubMed link.

Humphries, J. D., Schofield, N. R., Mostafavi-Pour, Z., Green, L. J., Garratt, A. N., Mould, A. P., and Humphries, M. J. (2005) Dual functionality of the anti-beta1 integrin antibody, 12G10, exemplifies agonistic signalling from the ligand binding pocket of integrin adhesion receptors, J Biol Chem 280, 10234-43 PubMed link.

Glasner, J., Blum, H., Wehner, V., Stilz, H. U., Humphries, J. D., Curley, G. P., Mould, A. P., Humphries, M. J., Hallmann, R., Rollinghoff, M., and Gessner, A. (2005) A small molecule alpha 4 beta 1 antagonist prevents development of murine Lyme arthritis without affecting protective immunity, J Immunol 175, 4724-34 PubMed link.

Gibson, R. M., Craig, S. E., Heenan, L., Tournier, C., and Humphries, M. J. (2005) Activation of integrin alpha5beta1 delays apoptosis of Ntera2 neuronal cells, Mol Cell Neurosci 28, 588-98 PubMed link.

Ewan, R., Huxley-Jones, J., Mould, A. P., Humphries, M. J., Robertson, D. L., and Boot-Handford, R. P. (2005) The integrins of the urochordate Ciona intestinalis provide novel insights into the molecular evolution of the vertebrate integrin family, BMC evolutionary biology 5, 31 PubMed link.

Dallas, S. L., Sivakumar, P., Jones, C. J., Chen, Q., Peters, D. M., Mosher, D. F., Humphries, M. J., and Kielty, C. M. (2005) Fibronectin regulates latent transforming growth factor-beta (TGF beta) by controlling matrix assembly of latent TGF beta-binding protein-1, J Biol Chem 280, 18871-80 PubMed link.

Clark, K., Pankov, R., Travis, M. A., Askari, J. A., Mould, A. P., Craig, S. E., Newham, P., Yamada, K. M., and Humphries, M. J. (2005) A specific alpha5beta1-integrin conformation promotes directional integrin translocation and fibronectin matrix formation, J Cell Sci 118, 291-300 PubMed link.

Bella, J., and Humphries, M. J. (2005) Calpha-H…O = C hydrogen bonds contribute to the specificity of RGD cell-adhesion interactions, BMC structural biology 5, 4 PubMed link.

Zhang, X., Craig, S. E., Kirby, H., Humphries, M. J., and Moy, V. T. (2004) Molecular basis for the dynamic strength of the integrin alpha4beta1/VCAM-1 interaction, Biophys J 87, 3470-8 PubMed link.

Travis, M. A., van der Flier, A., Kammerer, R. A., Mould, A. P., Sonnenberg, A., and Humphries, M. J. (2004) Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylation, FEBS Lett 569, 185-90 PubMed link.

Mould, A. P., and Humphries, M. J. (2004) Regulation of integrin function through conformational complexity: not simply a knee-jerk reaction?, Curr Opin Cell Biol 16, 544-51 PubMed link.

Mould, A. P., and Humphries, M. J. (2004) Cell biology: adhesion articulated, Nature 432, 27-8 PubMed link.

Humphries, M. J., Travis, M. A., Clark, K., and Mould, A. P. (2004) Mechanisms of integration of cells and extracellular matrices by integrins, Biochem Soc Trans 32, 822-5 PubMed link.

Humphries, M. J. (2004) Monoclonal antibodies as probes of integrin priming and activation, Biochem Soc Trans 32, 407-11 PubMed link.

Bax, D. V., Messent, A. J., Tart, J., van Hoang, M., Kott, J., Maciewicz, R. A., and Humphries, M. J. (2004) Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells, J Biol Chem 279, 22377-86 PubMed link.

Barton, S. J., Travis, M. A., Askari, J. A., Buckley, P. A., Craig, S. E., Humphries, M. J., and Mould, A. P. (2004) Novel activating and inactivating mutations in the integrin beta1 subunit A domain, Biochem J 380, 401-7 PubMed link.

Mould, A. P., Barton, S. J., Askari, J. A., Craig, S. E., and Humphries, M. J. (2003) Role of ADMIDAS cation-binding site in ligand recognition by integrin alpha 5 beta 1, J Biol Chem 278, 51622-9 PubMed link.

Mould, A. P., Symonds, E. J., Buckley, P. A., Grossmann, J. G., McEwan, P. A., Barton, S. J., Askari, J. A., Craig, S. E., Bella, J., and Humphries, M. J. (2003) Structure of an integrin-ligand complex deduced from solution x-ray scattering and site-directed mutagenesis, The Journal of biological chemistry 278, 39993-9 PubMed link.

Mould, A. P., Barton, S. J., Askari, J. A., McEwan, P. A., Buckley, P. A., Craig, S. E., and Humphries, M. J. (2003) Conformational changes in the integrin beta A domain provide a mechanism for signal transduction via hybrid domain movement, J Biol Chem 278, 17028-35 PubMed link.

Mostafavi-Pour, Z., Askari, J. A., Parkinson, S. J., Parker, P. J., Ng, T. T., and Humphries, M. J. (2003) Integrin-specific signaling pathways controlling focal adhesion formation and cell migration, J Cell Biol 161, 155-67 PubMed link.

Humphries, M. J., Symonds, E. J., and Mould, A. P. (2003) Mapping functional residues onto integrin crystal structures, Curr Opin Struct Biol 13, 236-43 PubMed link.

Humphries, M. J., McEwan, P. A., Barton, S. J., Buckley, P. A., Bella, J., and Mould, A. P. (2003) Integrin structure: heady advances in ligand binding, but activation still makes the knees wobble, Trends Biochem Sci 28, 313-20 PubMed link.

Bax, D. V., Bernard, S. E., Lomas, A., Morgan, A., Humphries, J., Shuttleworth, C. A., Humphries, M. J., and Kielty, C. M. (2003) Cell adhesion to fibrillin-1 molecules and microfibrils is mediated by alpha 5 beta 1 and alpha v beta 3 integrins, J Biol Chem 278, 34605-16 PubMed link.

Whittard, J. D., Craig, S. E., Mould, A. P., Koch, A., Pertz, O., Engel, J., and Humphries, M. J. (2002) E-cadherin is a ligand for integrin alpha2beta1, Matrix Biol 21, 525-32 PubMed link.

Santas, A. J., Peterson, J. A., Halbleib, J. L., Craig, S. E., Humphries, M. J., and Peters, D. M. (2002) Alternative splicing of the IIICS domain in fibronectin governs the role of the heparin II domain in fibrillogenesis and cell spreading, J Biol Chem 277, 13650-8 PubMed link.

Parsons, M., Keppler, M. D., Kline, A., Messent, A., Humphries, M. J., Gilchrist, R., Hart, I. R., Quittau-Prevostel, C., Hughes, W. E., Parker, P. J., and Ng, T. (2002) Site-directed perturbation of protein kinase C- integrin interaction blocks carcinoma cell chemotaxis, Mol Cell Biol 22, 5897-911 PubMed link.

Mould, A. P., Askari, J. A., Barton, S., Kline, A. D., McEwan, P. A., Craig, S. E., and Humphries, M. J. (2002) Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domain, J Biol Chem 277, 19800-5 PubMed link.

Humphries, M. J. (2002) Insights into integrin-ligand binding and activation from the first crystal structure, Arthritis Res 4 Suppl 3, S69-78 PubMed link.

Bass, M. D., and Humphries, M. J. (2002) Cytoplasmic interactions of syndecan-4 orchestrate adhesion receptor and growth factor receptor signalling, Biochem J 368, 1-15 PubMed link.

Aquilina, A., Korda, M., Bergelson, J. M., Humphries, M. J., Farndale, R. W., and Tuckwell, D. (2002) A novel gain-of-function mutation of the integrin alpha2 VWFA domain, European journal of biochemistry / FEBS 269, 1136-44 PubMed link.

Stallmach, A., Giese, T., Pfister, K., Wittig, B. M., Kunne, S., Humphries, M., Zeitz, M., and Meuer, S. C. (2001) Activation of beta(1) integrins mediates proliferation and inhibits apoptosis of intestinal CD4-positive lymphocytes, Eur J Immunol 31, 1228-38 PubMed link.

Mostafavi-Pour, Z., Askari, J. A., Whittard, J. D., and Humphries, M. J. (2001) Identification of a novel heparin-binding site in the alternatively spliced IIICS region of fibronectin: roles of integrins and proteoglycans in cell adhesion to fibronectin splice variants, Matrix Biol 20, 63-73 PubMed link.

Humphries, M. J., and Mould, A. P. (2001) Structure. An anthropomorphic integrin, Science 294, 316-7 PubMed link.

Humphries, M. J. (2001) Cell adhesion assays, Mol Biotechnol 18, 57-61 PubMed link.

Hicks, M. S., O’Leary, V., Wilkin, M., Bee, S. E., Humphries, M. J., and Baron, M. (2001) DrhoGEF3 encodes a new Drosophila DH domain protein that exhibits a highly dynamic embryonic expression pattern, Development genes and evolution 211, 263-7 PubMed link.

Coe, A. P., Askari, J. A., Kline, A. D., Robinson, M. K., Kirby, H., Stephens, P. E., and Humphries, M. J. (2001) Generation of a minimal alpha5beta1 integrin-Fc fragment, J Biol Chem 276, 35854-66 PubMed link.

Tuckwell, D. S., Smith, L., Korda, M., Askari, J. A., Santoso, S., Barnes, M. J., Farndale, R. W., and Humphries, M. J. (2000) Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a functionally important region within alpha2beta1, Biochem J 350 Pt 2, 485-93 PubMed link.

Mould, A. P., Askari, J. A., and Humphries, M. J. (2000) Molecular basis of ligand recognition by integrin alpha 5beta 1. I. Specificity of ligand binding is determined by amino acid sequences in the second and third NH2-terminal repeats of the alpha subunit, J Biol Chem 275, 20324-36 PubMed link.

Lyon, M., Rushton, G., Askari, J. A., Humphries, M. J., and Gallagher, J. T. (2000) Elucidation of the structural features of heparan sulfate important for interaction with the Hep-2 domain of fibronectin, J Biol Chem 275, 4599-606 PubMed link.

Jackson, T., Blakemore, W., Newman, J. W., Knowles, N. J., Mould, A. P., Humphries, M. J., and King, A. M. (2000) Foot-and-mouth disease virus is a ligand for the high-affinity binding conformation of integrin alpha5beta1: influence of the leucine residue within the RGDL motif on selectivity of integrin binding, J Gen Virol 81, 1383-91 PubMed link.

Humphries, J. D., Askari, J. A., Zhang, X. P., Takada, Y., Humphries, M. J., and Mould, A. P. (2000) Molecular basis of ligand recognition by integrin alpha5beta 1. II. Specificity of arg-gly-Asp binding is determined by Trp157 OF THE alpha subunit, J Biol Chem 275, 20337-45 PubMed link.

Humphries, M. J. (2000) Integrin structure, Biochem Soc Trans 28, 311-39 PubMed link.

Humphries, M. J. (2000) Integrin cell adhesion receptors and the concept of agonism, Trends Pharmacol Sci 21, 29-32 PubMed link.

Clark, K., Newham, P., Burrows, L., Askari, J. A., and Humphries,M. J. (2000) Production of recombinant soluble human integrin alpha4beta1, FEBS Lett 471, 182-6 PubMed link.

Valles, S., Tsoi, C., Huang, W. Y., Wyllie, D., Carlotti, F., Askari, J. A., Humphries, M. J., Dower, S. K., and Qwarnstrom, E. E. (1999) Recruitment of a heparan sulfate subunit to the interleukin-1 receptor complex. Regulation by fibronectin attachment, J Biol Chem 274, 20103-9 PubMed link.

Sharma, A., Askari, J. A., Humphries, M. J., Jones, E. Y., and Stuart, D. I. (1999) Crystal structure of a heparin- and integrin-binding segment of human fibronectin, EMBO J 18, 1468-79 PubMed link.

Schofield, K. P., and Humphries, M. J. (1999) Identification of fibronectin IIICS variants in human bone marrow stroma, Blood 93, 410-1 PubMed link.

Ng, T., Shima, D., Squire, A., Bastiaens, P. I., Gschmeissner, S., Humphries, M. J., and Parker, P. J. (1999) PKCalpha regulates beta1 integrin-dependent cell motility through association and control of integrin traffic, EMBO J 18, 3909-23 PubMed link.

Ng, T. T., Collins, I. E., Kanner, S. B., Humphries, M. J., Amft, N., Wickremasinghe, R. G., D’Cruz, D., Nye, K. E., and Morrow, W. J. (1999) Integrin signalling defects in T-lymphocytes in systemic lupus erythematosus, Lupus 8, 39-51 PubMed link.

McCormick, C. J., Tuckwell, D. S., Crisanti, A., Humphries, M. J., and Hollingdale, M. R. (1999) Identification of heparin as a ligand for the A-domain of Plasmodium falciparum thrombospondin-related adhesion protein, Mol Biochem Parasitol 100, 111-24 PubMed link.

Mastrangelo, A. M., Homan, S. M., Humphries, M. J., and LaFlamme, S. E. (1999) Amino acid motifs required for isolated beta cytoplasmic domains to regulate ‘in trans’ beta1 integrin conformation and function in cell attachment, J Cell Sci 112 ( Pt 2), 217-29 PubMed link.

Humphries, M. J. (1999) Towards a structural model of an integrin, Biochem Soc Symp 65, 63-78 PubMed link.

Curley, G. P., Blum, H., and Humphries, M. J. (1999) Integrin antagonists, Cell Mol Life Sci 56, 427-41 PubMed link.

Burrows, L., Clark, K., Mould, A. P., and Humphries, M. J. (1999) Fine mapping of inhibitory anti-alpha5 monoclonal antibody epitopes that differentially affect integrin-ligand binding, Biochem J 344 Pt 2, 527-33 PubMed link.

Watson, R. E., Parry, E. J., Humphries, J. D., Jones, C. J., Polson, D. W., Kielty, C. M., and Griffiths, C. E. (1998) Fibrillin microfibrils are reduced in skin exhibiting striae distensae, Br J Dermatol 138, 931-7 PubMed link.

Schofield, K. P., Humphries, M. J., de Wynter, E., Testa, N., and Gallagher, J. T. (1998) The effect of alpha4 beta1-integrin binding sequences of fibronectin on growth of cells from human hematopoietic progenitors, Blood 91, 3230-8 PubMed link.

Retta, S. F., Balzac, F., Ferraris, P., Belkin, A. M., Fassler, R., Humphries, M. J., De Leo, G., Silengo, L., and Tarone, G. (1998) beta1-integrin cytoplasmic subdomains involved in dominant negative function, Mol Biol Cell 9, 715-31 PubMed link.

Newham, P., Craig, S. E., Clark, K., Mould, A. P., and Humphries, M. J. (1998) Analysis of ligand-induced and ligand-attenuated epitopes on the leukocyte integrin alpha4beta1: VCAM-1, mucosal addressin cell adhesion molecule-1, and fibronectin induce distinct conformational changes, J Immunol 160, 4508-17 PubMed link.

Mould, A. P., Garratt, A. N., Puzon-McLaughlin, W., Takada, Y., and Humphries, M. J. (1998) Regulation of integrin function: evidence that bivalent-cation-induced conformational changes lead to the unmasking of ligand-binding sites within integrin alpha5 beta1, Biochem J 331 ( Pt 3), 821-8 PubMed link.

Mould, A. P., Burrows, L., and Humphries, M. J. (1998) Identification of amino acid residues that form part of the ligand-binding pocket of integrin alpha5 beta1, J Biol Chem 273, 25664-72 PubMed link.

Messent, A. J., Tuckwell, D. S., Knauper, V., Humphries, M. J., Murphy, G., and Gavrilovic, J. (1998) Effects of collagenase-cleavage of type I collagen on alpha2beta1 integrin-mediated cell adhesion, J Cell Sci 111 ( Pt 8), 1127-35 PubMed link.

McCutcheon, J. C., Hart, S. P., Canning, M., Ross, K., Humphries, M. J., and Dransfield, I. (1998) Regulation of macrophage phagocytosis of apoptotic neutrophils by adhesion to fibronectin, J Leukoc Biol 64, 600-7 PubMed link.

Humphries, M. J., and Newham, P. (1998) The structure of cell-adhesion molecules, Trends Cell Biol 8, 78-83 PubMed link.

Green, L. J., Mould, A. P., and Humphries, M. J. (1998) The integrin beta subunit, Int J Biochem Cell Biol 30, 179-84 PubMed link.

Globus, R. K., Doty, S. B., Lull, J. C., Holmuhamedov, E., Humphries, M. J., and Damsky, C. H. (1998) Fibronectin is a survival factor for differentiated osteoblasts, J Cell Sci 111 ( Pt 10), 1385-93 PubMed link.

Fernandez, C., Clark, K., Burrows, L., Schofield, N. R., and Humphries, M. J. (1998) Regulation of the extracellular ligand binding activity of integrins, Front Biosci 3, d684-700 PubMed link.

Tuckwell, D. S., Xu, Y., Newham, P., Humphries, M. J., and Volanakis, J. E. (1997) Surface loops adjacent to the cation-binding site of the complement factor B von Willebrand factor type A module determine C3b binding specificity, Biochemistry 36, 6605-13 PubMed link.

Tuckwell, D. S., and Humphries, M. J. (1997) A structure prediction for the ligand-binding region of the integrin beta subunit: evidence for the presence of a von Willebrand factor A domain, FEBS Lett 400, 297-303 PubMed link.

Tselepis, V. H., Green, L. J., and Humphries, M. J. (1997) An RGD to LDV motif conversion within the disintegrin kistrin generates an integrin antagonist that retains potency but exhibits altered receptor specificity. Evidence for a functional equivalence of acidic integrin-binding motifs, J Biol Chem 272, 21341-8 PubMed link.

Schofield, K. P., Rushton, G., Humphries, M. J., Dexter, T. M., and Gallagher, J. T. (1997) Influence of interleukin-3 and other growth factors on alpha4beta1 integrin-mediated adhesion and migration of human hematopoietic progenitor cells, Blood 90, 1858-66 PubMed link.

Ng, T. T., Kanner, S. B., Humphries, M. J., Wickremasinghe, R. G., Nye, K. E., Anderson, J., Khoo, S. H., and Morrow, W. J. (1997) The integrin-triggered rescue of T lymphocyte apoptosis is blocked in HIV-1-infected individuals, J Immunol 158, 2984-99 PubMed link.

Newham, P., Craig, S. E., Seddon, G. N., Schofield, N. R., Rees, A., Edwards, R. M., Jones, E. Y., and Humphries, M. J. (1997) Alpha4 integrin binding interfaces on VCAM-1 and MAdCAM-1. Integrin binding footprints identify accessory binding sites that play a role in integrin specificity, J Biol Chem 272, 19429-40 PubMed link.

Mould, A. P., Askari, J. A., Aota, S., Yamada, K. M., Irie, A., Takada, Y., Mardon, H. J., and Humphries, M. J. (1997) Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit, J Biol Chem 272, 17283-92 PubMed link.

Tuckwell, D.S. and Humphries, M.J. (1997) Ligand binding sites within the integrins. In: Integrin-Ligand Interaction, Eble, J.A. and Kühn, K., eds., Landes Bioscience, Austin, TX, pp. 199-217

Davies, D., Tuckwell, D.S., Calderwood, D.A., Weston, S.A., Takigawa, M. and Humphries, M.J. (1997) Molecular characterisation of integrin-procollagen C-propeptide interactions. Eur. J. Biochem. 246: 274-282

Lalor, P.F., Clements, J.M., Pigott, R., Humphries, M.J., Spragg, J.H. and Nash, G.B. (1997) Association between receptor density, cellular activation, and transformation of adhesive behaviour of flowing lymphocytes binding to VCAM-1. Eur. J. Immunol. 27: 1422-1426

Beauvais-Jouneau, A., Delouvée, A., Craig, S.E., Humphries, M.J., Thiery, J.-P. and Dufour, S. (1997) Direct role of the carboxy-terminal cell-binding domain of fibronectin in neural crest cell motility. Exp. Cell Res. 233: 1-10

Barry, S.T., Flinn, H.M., Humphries, M.J., Critchley, D.R. and Ridley, A.J. (1997) Requirement for Rho in integrin signalling. Cell Adhesion Commun. 4: 387-398

Calderwood, D.A., Tuckwell, D.S., Eble, J., Kühn, K. and Humphries, M.J. (1997) The integrin α1 A-domain is a ligand binding site for collagens and laminin. J. Biol. Chem. 272: 12311-12317

Humphries, M.J. (1996) Integrin activation: the link between ligand binding and signal transduction. Curr. Opinion Cell Biol. 8: 632-640

Newham, P. and Humphries, M.J. (1996) Integrin adhesion receptors: Structure, function and implications for biomedicine. Molecular Medicine Today 2: 304-313

Tuckwell, D. and Humphries, M. (1996) Integrin-collagen binding. Seminars Cell Dev. Biol. 7: 649-657

Mould, A.P. and Humphries, M.J. (1996) Functional domains of adhesion molecules. In: Adhesion Receptors as Therapeutic Targets, Horton, M.A., ed., CRC Press, Boca Raton, FL, pp. 75-105

Tuckwell, D.S., Reid, K.B.M., Barnes, M.J. and Humphries, M.J. (1996) The A-domain of integrin α2 binds specifically to a range of collagens but is not a general receptor for the collagenous motif. Eur. J. Biochem. 241: 732-739

Schor, S.L., Ellis, I., Dolman, C., Banyard, J., Humphries, M.J., Mosher, D.F., Grey, A.M., Mould, A.P., Sottile, J. and Schor, A.M. (1996) Substratum-dependent stimulation of fibroblast migration by the gelatin-binding domain of fibronectin. J. Cell Sci. 109: 2581-2590

Mould, A.P., Akiyama, S.K. and Humphries, M.J. (1996) The inhibitory anti-β1 integrin monoclonal antibody 13 recognizes an epitope that is attenuated by ligand occupancy: Evidence for allosteric inhibition of integrin function. J. Biol. Chem. 271: 20365-20374

Chambers, M., Dougan, G., Newman, J., Brown, F., Crowther, J., Mould, A.P., Humphries, M.J., Francis, M.J., Clarke, B., Brown, A.L. and Rowlands, D. (1996) Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions. J. Virol. 70: 4045-4052

Frankel, G., Lider, O., Hershkoviz, R., Mould, A.P., Kachalsky, S.G., Candy, D.C.A., Cahalon, L., Humphries, M.J. and Dougan, G. (1996) The cell binding domain of intimin from enteropathogenic Escherichia coli binds to β1 integrins. J. Biol. Chem. 271: 20359-20364

Hukins, D.W.L., Weston, S.A., Humphries, M.J. and Freemont, A.J. (1995) Cellular organelles and the extracellular matrix. In: Principles of Medical Biology, Vol. 3, Bittar, E.E. and Bittar, N., eds., JAI Press, Greenwich, CT, pp. 181-232

Humphries, M.J. (1995) The LDV peptide motif: Its role in integrin-ligand binding. In: Adhesion Molecules and Cell Signalling: Biology and Clinical Applications, Topics in Molecular Medicine, Vol. 1, Siess, R., Lorenz, R. and Weber, P.C., eds., Raven Press, pp. 27-35

Garratt, A.N. and Humphries, M.J. (1995) Recent insights into ligand binding, activation and signalling by integrin adhesion receptors. Acta Anatomica 154: 34-45

Humphries, M.J., Sheridan, J., Mould, A.P. and Newham, P. (1995) Mechanisms of VCAM-1 and fibronectin binding to integrin α4β1: Implications for integrin function and rational drug design. Ciba Foundation Symp. 189: 177-194

Tuckwell, D.S., Humphries, M.J. and Brass, A. (1995) Protein secondary structure prediction by the analysis of variation and conservation in multiple alignments. Comput. Applied Biosci. 11: 627-632

Altevogt, P., Hubbe, M., Ruppert, M., Lohr, J., von Hoegen, P., Sammar, M., Andrew, D.P., McEvoy, L., Humphries, M.J. and Butcher, E.C. (1995) The α4 integrin chain is a ligand for α4β7 and α4β1. J. Exp. Med. 182: 345-355

Mould, A.P., Akiyama, S.K. and Humphries, M.J. (1995) Regulation of integrin α5β1-fibronectin interactions by divalent cations: Evidence for distinct classes of binding sites for Mn2+, Mg2+ and Ca2+. J. Biol. Chem. 270: 26270-26277

Huhtala, P., Humphries, M.J., McCarthy, J.B., Tremble, P.M., Werb, Z. and Damsky, C.H. (1995) Cooperative signaling by α5β1 and α4β1 integrins regulates metalloproteinase gene expression in fibroblasts adhering to fibronectin. J. Cell Biol. 129: 867-879

Mould, A.P., Garratt, A.N., Askari, J.A., Akiyama, S.K. and Humphries, M.J. (1995) Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the β1 subunit. FEBS Lett. 363: 118-122

Tuckwell, D., Calderwood, D.A., Green, L.J. and Humphries, M.J. (1995) Integrin α2I-domain is a binding site for collagens. J. Cell Sci. 108: 1629-1637

Weston, S.A., Crossett, B., Tuckwell, D.S. and Humphries, M.J. (1995) Effect of β-mercaptoethanol on the detection of biotinylated proteins. Anal. Biochem. 225: 28-33

Beauvais, A., Erickson, C.A., Goins, T., Craig, S.E., Humphries, M.J., Thiery, J.P. and Dufour, S. (1995) Changes in the fibronectin-specific integrin expression pattern modify the migratory behavior of sarcoma S180 cells in vitro and in the embryonic environment. J. Cell Biol. 128: 699-713

Humphries, M.J. (1994) Mechanisms of ligand binding by integrins. Biochem. Soc. Trans. 22: 275-282

Humphries, M.J., Doyle, P.M. and Harris, C.J. (1994) Integrin antagonists as modulators of adhesion. Expert Opinion Therapeutic Patents 4: 227-235

Dudgeon, T.J., Bottomley, M.J., Driscoll, P.C., Humphries, M.J., Mould, A.P., Wingfield, G.I. and Clements, J.M. (1994) Expression and characterisation of a very-late antigen-4 (α4β1) integrin-binding fragment of vascular cell adhesion molecule-1. Eur. J. Biochem. 226: 517-523

Tuckwell, D.S., Humphries, M.J. and Brass, A.M. (1994) A secondary structure model of the integrin α subunit EF-hand domain based on analysis of multiple alignments. Cell Adhesion Commun. 2: 385-402

Humphries, M.J., Mould, A.P. and Weston, S.A. (1994) Conjugation of synthetic peptides to carrier proteins for cell adhesion studies. J. Tissue Culture Methods 16: 239-242

Mould, A.P., Askari, J.A., Craig, S.E., Garratt, A.N., Clements, J. and Humphries, M.J. (1994) Integrin α4β1-mediated melanoma cell adhesion and migration on vascular cell adhesion molecule-1 (VCAM-1) and the alternatively spliced IIICS region of fibronectin. J. Biol. Chem. 269: 27224-27230

Weston, S.A., Hulmes, D.J.S., Mould, A.P., Watson, R.B. and Humphries, M.J. (1994) Identification of the integrin α2β1 as a cell surface receptor for the C-propeptide of type I procollagen. J. Biol. Chem. 269: 20982-20986

Clements, J.M., Newham, P., Shepherd, M., Gilbert, R., Dudgeon, T.J., Needham, L.A., Edwards, R.M., Berry, L., Brass, A. and Humphries, M.J. (1994) Identification of a key integrin-binding sequence in VCAM-1 homologous to the LDV active site in fibronectin. J. Cell Sci. 107: 2127-2135

Tuckwell, D.S., Ayad, S., Grant, M.E., Takigawa, M. and Humphries, M.J. (1994) Conformation-dependence of integrin-type II collagen binding: Inability of collagen peptides to support α2β1 binding, and mediation of adhesion to denatured collagen by a novel α5β1-fibronectin bridge. J. Cell Sci. 107: 993-1005

Morton, L.F., Peachey, A.R., Zijenah, L.S., Goodall, A.H., Humphries, M.J. and Barnes, M.J. (1994) Conformation-dependent platelet adhesion to collagen involving integrin α2β1-mediated and other mechanisms: Multiple α2β1-recognition sites in collagen type I. Biochem. J. 299: 791-797

Makarem, R., Newham, P., Askari, J.A., Green, L.J., Clements, J., Edwards, M., Humphries, M.J. and Mould, A.P. (1994) Competitive binding of vascular cell adhesion molecule-1 and the HepII/IIICS domain of fibronectin to the integrin α4β1. J. Biol. Chem. 269: 4005-4011

Ayad, S., Boot-Handford, R.P., Humphries, M.J., Kadler, K.E. and Shuttleworth, C.A. (1993) The Extracellular Matrix FactsBook, Academic Press, London

Tuckwell, D.S., Weston, S.A. and Humphries, M.J. (1993) Integrins: A review of their structure and mechanisms of ligand binding. In: Cell Behaviour: Adhesion and Motility, Soc. Exp. Biol. Symp. Vol. 47, Jones, G., Wigley, C. and Warn, R., eds., Society for Experimental Biology/Company of Biologists, Cambridge, pp. 107-136

Humphries, M.J. (1993) Peptide sequences in matrix proteins recognized by adhesion receptors. In: Molecular and Cellular Aspects of Basement Membranes, Rohrbach, D.H. and Timpl, R., eds., Academic Press, Inc., New York, NY, pp. 289-308

Tuckwell, D.S. and Humphries, M.J. (1993) The molecular and cellular biology of integrins. CRC Crit. Rev. Oncol. Hematol. 15: 149-171

Humphries, M.J. (1993) Fibronectin and cancer: Rationales for the use of antiadhesives in cancer treatment. Seminars Cancer Biol. 4: 293-299

Humphries, M.J., Mould, A.P. and Tuckwell, D.S. (1993) Dynamic aspects of adhesion receptor function – Integrins both twist and shout. Bioessays 15: 391-397

Humphries, M.J. and Mould, A.P. (1993) Integrin adhesion receptors in epidermal wound healing. Wounds 5: 24-31

Humphries, M.J. (1993) Putting cells in their place. Biol. Sci. Rev. 5: 7-9

May, M.J., Entwistle, G., Humphries, M.J. and Ager, A. (1993) VCAM-1 is a CS1 peptide-inhibitable adhesion molecule expressed by lymph node endothelium. J. Cell Sci. 106: 109-119

Santoni, A., Gismondi, A., Palmieri, G., Morrone, S., Mainiero, F., Santoni, G., Humphries, M.J., Piccoli, M. and Frati, L. (1992) Fibronectin receptors on NK cells. In: NK Cell Mediated Cytotoxicity, Lotzova, E. and Herberman, R.B., eds., CRC Press, Boca Raton, FL, pp. 85-96

Humphries, M.J. (1992) Peptide recognition motifs involved in the binding of integrins to their ligands and implications for the generation of adhesive specificity. Kidney Int. 41: 645-649

Rodriguez, R.M., Pitzalis, C., Kingsley, G.H., Henderson, E., Humphries, M.J. and Panayi, G.S. (1992) T lymphocyte adhesion to fibronectin: A possible mechanism for T cell accumulation in the rheumatoid joint. Clin. Exp. Immunol. 89: 439-445

Yoshihara, T., Esumi, N., Humphries, M.J. and Imashuku, S. (1992) Unique expression of integrin fibronectin receptors in human neuroblastoma cell lines. Int. J. Cancer 51: 620-626

Tuckwell, D.S., Brass, A. and Humphries, M.J. (1992) Homology modelling of integrin EF-hands. Evidence for widespread use of a conserved cation-binding site. Biochem. J. 285: 325-331

Szekanecz, Z., Humphries, M.J. and Ager A. (1992) Lymphocyte adhesion to high endothelium is mediated by two 1 integrin receptors for fibronectin, α4β1 and α5β1. J. Cell Sci. 101: 885-894

Humphries, M.J., Mould, A.P. and Yamada, K.M. (1991) Matrix receptors in cell migration. In: Receptors for Extracellular Matrix, McDonald, J.A. and Mecham, R.P., eds., Academic Press, Inc., New York, NY, pp. 195-253

Olden, K., White, S.L., Newton, S.A., Molyneux, R.J. and Humphries, M.J. (1991) Antineoplastic potential and other possible uses of swainsonine and related compounds. In: Handbook of Natural Toxins, Vol. 6. Toxicology of Plant and Fungal Compounds, Keeler, R.F. and Tu, A.T., eds., Marcel-Dekker, New York, NY, pp. 575-588

Ager, A. and Humphries, M.J. (1991) Integrin α4β1: Its structure, ligand-binding specificity and role in lymphocyte-endothelial cell interactions. Chemical Immunol. 50: 55-74

Mould, A.P. and Humphries, M.J. (1991) Identification of a novel recognition sequence for the integrin α4β1 in the COOH-terminal heparin-binding domain of fibronectin. EMBO J. 10: 4089-4095

Yoshihara, T., Ikushima, S., Shimizu, Y., Esumi, N., Todo, S., Humphries, M.J. and Imashuku, S. (1991) Distinct mechanism of human neuroblastoma cell adhesion to fibronectin. Clin. Exp. Metastasis 9: 363-375

Komoriya, A., Green, L.J., Mervic, M., Yamada, S.S., Yamada, K.M. and Humphries, M.J. (1991) The minimal essential sequence for a major cell-type specific adhesion site (CS1) within the alternatively spliced IIICS domain of fibronectin is Leu-Asp-Val. J. Biol. Chem. 266: 15075-15079

Mould, A.P., Komoriya, A., Yamada, K.M. and Humphries, M.J. (1991) The CS5 peptide is a second site in the IIICS region of fibronectin recognized by the integrin α4β1. Inhibition of α4β1 function by RGD peptide homologues. J. Biol. Chem. 266: 3579-3585

Gismondi, A., Morrone, S., Humphries, M.J., Piccoli, M., Frati, L. and Santoni, A. (1991) Human natural killer cells express VLA-4 and VLA-5 which mediate their adhesion to fibronectin. J. Immunol. 146: 384-392

Humphries, M.J. and Yamada, K.M. (1990) Cell interaction sites of fibronectin in adhesion and metastasis. In: Morphoregulatory Molecules, Edelman, G.M., Cunningham, B.A. and Thiery, J.P., eds., John Wiley & Sons, Inc., New York, NY, pp. 137-172

Humphries, M.J. (1990) The molecular basis and specificity of integrin-ligand interactions. J. Cell Sci. 97: 585-592

Nojima, Y., Humphries, M.J., Mould, A.P., Komoriya, A., Yamada, K.M., Schlossman, S.F. and Morimoto, C. (1990) VLA-4 mediates CD3-dependent CD4+ T cell activation via the CS1 alternatively spliced domain of fibronectin. J. Exp. Med. 172: 1185-1192

Ager, A. and Humphries, M.J. (1990) Use of synthetic peptides to probe lymphocyte-high endothelial cell interactions. Lymphocytes recognise a ligand on the endothelial surface which contains the CS1 adhesion motif. Int. Immunol. 2: 921-928

Mould, A.P., Wheldon, L.A., Komoriya, A., Wayner, E.A., Yamada, K.M. and Humphries, M.J. (1990) Affinity chromatographic isolation of the melanoma adhesion receptor for the IIICS region of fibronectin and its identification as the integrin α4β1. J. Biol. Chem. 265: 4020-4024

Humphries, M.J., Matsumoto, K., White, S.L., Molyneux, R.J. and Olden, K. (1990) An assessment of the effects of swainsonine on survival of mice injected with B16-F10 melanoma cells. Clin. Exp. Metastasis 8: 89-102

Olden, K., White, S.L., Matsumoto, K. and Humphries, M.J. (1989) Investigation of the possible involvement of glycoprotein glycans in experimental metastasis of B16-F10 murine melanoma cells. In: Swainsonine and Related Glycosidase Inhibitors, James, L.F., Elbein, A.D., Molyneux, R.J. and Warren, C.D., eds., Iowa State University Press, Ames, IA, pp. 445-465

White, S.L., Humphries, M.J., Molyneux, R.J. and Olden, K. (1989) Swainsonine: A new immunomodulator which enhances murine lymphoproliferation and natural killer activity. In: Swainsonine and Related Glycosidase Inhibitors, James, L.F., Elbein, A.D., Molyneux, R.J. and Warren, C.D., eds., Iowa State University Press, Ames, IA, pp. 425-444

Humphries, M.J., Olden, K. and Yamada, K.M. (1989) Fibronectin and cancer: Implications of cell adhesion to fibronectin for tumor metastasis. In: Fibronectin in Health and Disease, Carsons, S.E., ed., CRC Press Inc., Boca Raton, FL, pp. 161-199

Olden, K., White, S.L., Mohla, S., Newton, S.A., Yasuda, Y., Bowen, D. and Humphries, M.J. (1989) Experimental approaches for the prevention of hematogenous metastasis. Oncology 3: 83-91

Humphries, M.J., Obara, M., Olden, K. and Yamada, K.M. (1989) Role of fibronectin in adhesion, migration, and metastasis. Cancer Invest. 7: 373-393

Humphries, M.J. and Olden, K. (1989) Asparagine-linked oligosaccharides and tumor metastasis. Pharmacol. Therapeutics 44: 85-105

Mohla, S., Humphries, M.J., White, S.L., Matsumoto, K., Newton, S.A., Sampson, C.C., Bowen, D. and Olden, K. (1989) Swainsonine: A new antineoplastic immunomodulator. J. Natl. Med. Assn. 81: 1049-1056

Wayner, E.A., Garcia-Pardo, A., Humphries, M.J., McDonald, J.A. and Carter, W.G. (1989) Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J. Cell Biol. 109: 1321-1330

Newton, S.A., White, S.L., Humphries, M.J. and Olden, K. (1989) Swainsonine inhibition of spontaneous metastasis. J. Natl. Cancer Inst. 81: 1024-1028

Humphries, M.J., Komoriya, A., Akiyama, S.K., Olden, K. and Yamada, K.M. (1988) Identification of two distinct regions of an alternatively spliced site in human plasma fibronectin that promotes cell-type specific adhesion. In: Proceedings of the 10th American Peptide Symposium, Marshall, G.R., ed., ESCOM, Leiden, pp. 632-634

Olden, K., Mohla, S., Newton, S.A., White, S.L. and Humphries, M.J. (1988) Use of antiadhesive peptide and swainsonine to inhibit metastasis. Ann. N.Y. Acad. Sci. USA 551: 421-442

Humphries, M.J., Yasuda, Y., Olden, K. and Yamada, K.M. (1988) The cell interaction sites of fibronectin in tumour metastasis. Ciba Found. Symp. 141: 75-93

Dufour, S., Duband, J.-L., Humphries, M.J., Obara, M., Yamada, K.M. and Thiery, J.P. (1988) Attachment, spreading and locomotion of avian neural crest cells are mediated by multiple adhesion sites on fibronectin molecules. EMBO J. 7: 2661-2671

Humphries, M.J., Akiyama, S.K., Komoriya, A., Olden, K. and Yamada, K.M. (1988) Neurite extension of chicken peripheral nervous system neurons on fibronectin: Relative importance of specific adhesion sites in the central cell-binding domain and the alternatively spliced type III connecting segment. J. Cell Biol. 106: 1289-1297

White, S.L., Schweitzer, K., Humphries, M.J. and Olden, K. (1988) Stimulation of DNA synthesis in murine lymphocytes by the drug swainsonine: Immunomodulatory properties. Biochem. Biophys. Res. Commun. 150: 615-625

Humphries, M.J., Matsumoto, K., White, S.L., Molyneux, R.J. and Olden, K. (1988) Augmentation of murine natural killer cell activity by swainsonine, a new antimetastatic immunomodulator. Cancer Res. 48: 1410-1415

Humphries, M.J., Yamada, K.M. and Olden, K. (1988) Investigation of the biological effects of anti-cell adhesive synthetic peptides that inhibit experimental metastasis of B16-F10 murine melanoma cells. J. Clin. Invest. 81: 782-790

Humphries, M.J., Matsumoto, K., White, S.L. and Olden, K. (1987) Investigation of the antimetastatic effects of agents that inhibit cell adhesion or protein glycosylation. J. Natl. Med. Assn. 79: 411-419

Humphries, M.J., Komoriya, A., Akiyama, S.K., Olden, K. and Yamada, K.M. (1987) Identification of two distinct regions of the type III connecting segment of human plasma fibronectin that promote cell type-specific adhesion. J. Biol. Chem. 262: 6886-6892

Yamada, K.M., Akiyama, S.K., Humphries, M.J., Yamada, S.S. and Santoro, S.A. (1986) Platelet and fibroblast interactions with fibronectin and other adhesive proteins. In: Biology and Pathology of Platelet-Vessel Wall Interactions, Jolles, G., Legrand, Y.J. and Nurden, A., eds., Academic Press Inc., London, pp. 53-74

Humphries, M.J. and Yamada, K.M. (1986) Non-collagenous glycoproteins. In: Rheumatology. An Annual Review, Vol. 10. Connective Tissue: Biological and Clinical Aspects, Kühn, K. and Krieg, T., eds., S. Karger A.G., Basel, pp. 104-142

Humphries, M.J., Akiyama, S.K., Komoriya, A., Olden, K. and Yamada, K.M. (1986) Identification of an alternatively spliced site in human plasma fibronectin that mediates cell type-specific adhesion. J. Cell Biol. 103: 2637-2647

Humphries, M.J., Matsumoto, K., White, S.L. and Olden, K. (1986) Inhibition of experimental metastasis by castanospermine in mice: Blockage of two distinct stages of tumor colonization by oligosaccharide processing inhibitors. Cancer Res. 46: 5215-5222

Humphries, M.J., Olden, K. and Yamada, K.M. (1986) A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science 233: 467-470

Humphries, M.J., Matsumoto, K., White, S.L. and Olden, K. (1986) Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells. Proc. Natl. Acad. Sci. USA 83: 1752-1756

Yamada, K.M., Humphries, M.J., Hasegawa, T., Hasegawa, E., Olden, K., Chen, W.-T. and Akiyama, S.K. (1985) Fibronectin: Molecular approaches to analyzing cell interactions with the extracellular matrix. In: The Cell in Contact. Adhesions and Junctions as Morphogenetic Determinants, Edelman, G.M. and Thiery, J.-P., eds., John Wiley & Sons, Inc., New York, NY, pp. 303-332

Olden, K., Humphries, M.J. and White, S.L. (1985) Biochemical effects and cancer therapeutic potential of tunicamycin. In: Monoclonal Antibodies and Cancer Therapy, UCLA Symposia on Molecular and Cell Biology, New Series, Vol. 27, Reisfeld, R.A. and Sell, S., eds., Alan R. Liss, Inc., New York, NY, pp. 443-472

Yamada, K.M., Akiyama, S.K., Hasegawa, T., Hasegawa, E., Humphries, M.J., Kennedy, D.W., Nagata, K., Urushihara, H., Olden, K. and Chen, W.-T. (1985) Recent advances in research on fibronectin and other cell attachment proteins. J. Cell. Biochem. 28: 79-97

Olden, K., Bernard, B.A., Humphries, M.J., Yeo, T.-K., Yeo, K.-T., White, S.L., Newton, S.A., Bauer, H.C. and Parent, J.B. (1985) Function of glycoprotein glycans. Trends Biochem. Sci. 10: 78-82

Nagata, K., Humphries, M.J., Olden, K. and Yamada, K.M. (1985) Collagen can modulate cell interactions with fibronectin. J. Cell Biol. 101: 386-394

Humphries, M.J. and Ayad, S.R. (1983) Stimulation of DNA synthesis by cathepsin D digests of fibronectin. Nature 305: 811-813

Publications – right hand column

Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.